水氮调控对苜蓿与无芒雀麦混播草地生长和辐射利用的影响

针对我国内陆干旱区人工草地生产管理粗放及气候资源利用不充分等问题,探究合理的牧草种植与水氮供应模式,以充分挖掘区域牧草的生产潜力。采用3 a生(2018年播种)紫花苜蓿(简称‘苜蓿’)和无芒雀麦,分析种植模式(苜蓿与无芒雀麦混播,D1;无芒雀麦单播,D2)、施氮量(低氮量N1:60 kg·hm^-2;高氮量N2:120 kg·hm^-2)和灌水量(以灌水下限占田间持水量θ_f的百分比计,分枝期均充分灌水(75%～85%θ_f),现蕾和初花期轻度亏水W1:65%～75%θ_f、中度亏水W2:55%～65%θ_f、重度亏水W3:45%～55%θ_f,灌水上限均为85%θ_f)对牧草叶面积指数(LAI)、干物质累积量、累积截获光合有效辐射量(CIPAR)、辐射利用效率(RUE)、产量(Y)、耗水量(ET_a)、水分利用效率(WUE)和氮肥偏生产力(PFP_N)的影响。结果表明：(1)牧草LAI和...     

Effects of utilization of local food by‐products as total mixed ration silage materials on fermentation quality and intake,digestibility, rumen condition and nitrogen availability in sheep

Four wethers were used in a 4 × 4 Latin square design experiment to evaluate in vivo digestibility of total mixed ration (TMR) silage with food by‐products for dairy cows, and the ruminal condition and nitrogen (N) balance were examined. Five by‐products (i.e. potato waste, noodle waste, soybean curd residue, soy sauce cake and green tea waste) were obtained. Four types of TMR silage were used: control (C) containing roughage and commercial concentrate, T1:20% and T1:40% containing the five by‐products replacing 20% and 40% of the commercial concentrate on a dry matter (DM) basis, respectively, and T2:40% containing three by‐products (potato waste, noodle waste and soybean curd residue) replacing 40% of the commercial concentrate on a DM basis. The ingredients were mixed and preserved in oil drum silos for 4 months. The TMR silages showed 4.02–4.44% and 1.75–2.19% for pH and lactic acid contents, respectively. The digestibility of DM and neutral detergent fiber, and total digestible nutrient content were higher (P < 0.05) for T2:40% feeding than for C feeding. Urinary nitrogen excretion tended to be lower (P = 0.07) for T2:40% than for C. The results suggested 40% replacing of commercial concentrate by using the three food by‐products can be most suitable for TMR silage.     

绿洲区苜蓿与鸭茅混播饲草的青贮发酵品质研究

以苜蓿(Medicago sativa)与鸭茅(Dactylis glomerata)混播草地牧草为材料,按照90%苜蓿+10%鸭茅、70%苜蓿+30%鸭茅、50%苜蓿+50%鸭茅的比例进行青贮,通过分析对比发酵品质、营养成分、感官青贮效果等,以期优化最佳混合青贮的豆∶禾比例。试验结果表明:苜蓿和鸭茅混合青贮后,气味、质地、色泽感官评定均为优良,且pH呈下降趋势,青贮效果优于苜蓿和鸭茅的单独青贮。混合青贮降低了苜蓿青贮中氨态氮含量的36.4%,鸭茅青贮中乙酸、丙酸的含量明显下降;提高了苜蓿青贮中乳酸的含量,有效抑制了丁酸的产生,苜蓿+鸭茅饲草混贮比例为7∶3和5∶5处理结果未检测出丁酸。3个混合青贮组合的粗蛋白含量高出鸭茅单贮31.9%,酸性洗涤纤维低于鸭茅青贮12.6%。在青贮发酵过程中,苜蓿+鸭茅以7∶3比例混合青贮,有利于提高青贮饲料的发酵品质。     

基于 GIS 的中国草地合理载畜量空间化

天然草地合理载畜量是反映草地合理利用强度的基本单位。为提高草地合理载畜量的划分精度,根据“极大似然逼近原理”,创建了异质因子控制下的“线性回归+区域化值残差修正”数据空间化方法。以全国草地资源调查汇总数据中县域理论载畜量和1∶400万中国草地资源图为基础数据,采用分省控制构建了理论载畜量与植被类型-生产力异质植被斑块的线性回归模型,其判定系数由全国模型下的0.867提高到0.937,拟合优势度较高,所获得的中国公里格网草地合理载畜量分布图空间化精度也较高。中国公里格网草地合理载畜量分布图不仅表明了不同类草地因生产力的不同有不同的放牧要求,也体现了不同环境条件下草地载畜能力的差别,有效反映了单位面积内草地植被载畜-生产力水平,为生态治理和草地资源保育工作中合理制定草地利用强度提供了理论参考。     

贵州某野生动物园长臂猿流感病毒、支原体及巴氏杆菌混合感染的诊治

为弄清贵州某野生动物园长臂猿死亡的原因,本试验采用流行病学调查、临床症状观察、病理剖检诊断和RT-PCR检测确诊等方法,对该野生动物园发病长臂猿进行了诊断。试验结果显示,流行病学调查、剖检病理初步诊断该野生动物园长臂猿疑似流感病毒、支原体和细菌混合感染,RT-PCR/PCR检测确诊发病长臂猿为流感病毒、支原体混合感染,细菌分离培养、生化特性鉴定和动物致病性试验确诊为多杀性巴氏杆菌感染。结果表明造成该野生动物园长臂猿发病死亡的原因为流感病毒、支原体和多杀性巴氏杆菌混合感染。根据诊断及药敏试验结果,制定出了免疫预防及治疗措施。     

Pathogen Analysis of Bovine Respiratory Disease Complex

To investigate the pathogen of bovine respiratory disease complex (BRDC) of a dairy farm in Guangxi, a strain of Mycoplasma and a strain of gram-negative pathogenic bacterium were isolated and identified by the means of field surveys, clinic observation, pathological examination, isolation studies and so on.Treatments were taken according to drug sensitivity test results.The Mycoplasma strain, growing on PPLO medium, formed typical "fried egg" colonies.A 448 bp of oppF fragment was amplified by PCR from the strain and had 98.4% nucleotide identity with Mycoplasma bovis reference isolate PG5 of USA.The biochemical features of the gram-negative bacterial isolate were same with Serratia marcescens.The PCR amplified 16S rDNA of the gram-negative pathogenic bacterium strain was 1 400 bp.It shared 99.0% nucleotide identity with other Serratia marcescens reference strains obtained from GenBank.Animal experiment showed that the gram-negative pathogenic bacterium isolate could cause the mice to die.The drug sensitivity tests showed all isolates were sensitive to spectinomycin, azithromycin, amikacin, gentamicin and neomycin.It was effective to treat with dexamethasone and spectinomycin.Pathogen analysis and drug treatment showed that the BRDC was caused by Mycoplasma bovis and Serratia marcescens.     

One hundred years of population ecology: Successes,failures and the road ahead

Population ecology is the most mature of the three subdisciplines of ecology partly because it has a solid mathematical foundation and partly because it can address the primary questions of distribution and abundance with experimental protocols. Yet there is much left to do to integrate our population knowledge into community and ecosystem ecology to help address the global issues of food security and the conservation of biodiversity. Many different approaches are now being developed to bring about this integration and much more research will be necessary to decide which if any will be most useful in achieving our goals of explaining the changes we see in the distribution and abundance of animals and plants. Food web ecology would appear to be the best approach at present because it uses the detailed information of the population ecology of particular species in combination with data on consumer–resource interactions to apply to the applied problems of biodiversity conservation, food security, pest management and disease prevention. If we can use our understanding of population ecology to address the practical problems of our time in a creative way, we will benefit both the human population and the Earth's biodiversity. Much remains to be done.     

滇西北高寒地区牧草混播组合的筛选

为提高香格里拉高寒地区饲草生产能力,在小中甸镇开展了牧草混播组合试验.试验设5个混播组合(A1、A2、A3、A4、A5),随机区组设计,连续两年对牧草产量和组分进行测定,第2年取混合样对营养含量进行分析.结果表明,A1和A5组合牧草产量显著高于A2、A3和A4 (P＜0.05);A3、A1、A5年度间群落组分变化不大,稳定性较强;A2、A4、A5的粗蛋白含量较高,均超过24％.3项指标综合考虑,A1[安巴鸭茅(Dactylis glomerata cv.Anmba)+草地休衣白三叶(Trifolium repens cv.Grasslands huia)+红三叶(T.pratense)]和A5[安巴鸭茅+雅晴多年生黑麦草(Lolium perenne cv.Yatsyn)+海法白三叶(T.repens cv.Haifa)+普纳菊苣(Cichorium intybus cv.Puna)]适宜在香格里拉高寒地区种植应用.     

红外光谱结合多元线性回归法快速测定木塑复合材料中木粉含量

采用KBr压片法对杉木/聚丙烯(PP)复合材料样品进行了红外光谱分析,确定杉木特征吸收谱带为1740~1730、1610~1590、1270~1260、1060~1050以及1040~1030 cm-1,以PP在1377 cm-1处吸收强度(I)为内标,对木塑复合材料(WPC)中木粉含量和杉木特征峰相对吸收强度进行相关性分析,并采用逐步多元线性回归法建立木粉含量与相对峰强间的多元线性回归方程。结果表明,选取I(1060-1050)/I1377、I(1270-1260)/I1377为回归变量建立的二元线性回归方程和以I(1060-1050)/I1377、I(1040-1030)/I1377及I(1270-1260)/I1377为回归变量建立的三元线性回归方程,具有较高的预测精度。木粉含量的预测值和参照值之间具有强烈的相关性,校正决定系数(R2c)超过0.98,验证决定系数(R2p)超过0.96。外部验证结果表明,线性回归方程预测准确性较高,预测相对偏差范围为0.9%至7.4%,其中三元线性回归方程预测准确性稍好于二元线性回归方程。     

基于改进端元提纯模型的MODIS森林类型识别研究

MODIS遥感数据具有很高的光谱辐射精度,以及成本低、覆盖面积广、获取容易、周期短等数据特征,可以实现全覆盖大尺度区域森林类型信息快速提取,但由于其空间分辨率较低,遥感数据中存在混合像元。利用混合像元分解模型进行分解可得到较好的分类结果,但混合像元分解的端元组分直接影响分类的精度。利用决策树分类模型改进端元提纯,分析各地物的MODIS时间序列植被指数变化规律及物候变化规律,利用决策树模型分类的结果进行端元组分的提纯,最后进行混合像元分解。研究结果表明:分类精度最高的是线性混合像元分解,其次是最大似然分类,最差的是非线性混合像元分解,其中带约束和不带约束的线性分解模型的精度相差不大。